Genetic Diversity and DNA Fingerprinting in Broccoli Carrying Multiple Clubroot Resistance Genes Based on SSR Markers

نویسندگان

چکیده

To identify cultivars quickly and accurately, DNA fingerprinting of 10 broccoli varieties was performed by using simple sequence repeat (SSR) marker technology. Highly informative polymorphic SSR markers were screened rapeseed. Out the 93 pairs, 21 pairs selected found to have good polymorphism. Each pair generated 1 bands with an average 4.29. The polymorphism information content (PIC) 0.41 a range from 0.16 0.95. Six established accessions their unique fingerprints. Cluster analysis showed that genetic similarity coefficient between 0.57 0.91. They can be divided into 3 groups at (GSC) 0.73. above results indicated could provide scientific basis for identification polymerized multiple clubroot resistance genes. Research shows marker-based further ensures plant seed purity.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Use of EST-SSR markers for evaluating genetic diversity and fingerprinting celery (Apium graveolens L.) cultivars.

Celery (Apium graveolens L.) is one of the most economically important vegetables worldwide, but genetic and genomic resources supporting celery molecular breeding are quite limited, thus few studies on celery have been conducted so far. In this study we made use of simple sequence repeat (SSR) markers generated from previous celery transcriptome sequencing and attempted to detect the genetic d...

متن کامل

Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers

Durian (Durio zibethinus) is one of the most popular tropical fruits in Asia. To date, 126 durian types have been registered with the Department of Agriculture in Malaysia based on phenotypic characteristics. Classification based on morphology is convenient, easy, and fast but it suffers from phenotypic plasticity as a direct result of environmental factors and age. To overcome the limitation o...

متن کامل

Genetic diversity and genetic relationships of japonica rice varieties in Northeast Asia based on SSR markers

Genetic diversity and the relationship among nine japonica rice groups consisting of 288 landraces and varieties in different geographical origins of Northeast Asia (China, Japan, Korea, Democratic People's Republic of Korea) and the Russian Far East district of the Russian Federation were evaluated with 154 simple sequence repeat (SSR) markers. A total of 823 alleles were detected. The observe...

متن کامل

Dna Fingerprinting of Commercial Mushrooms by Issr and Ssr Markers for Genetic Discrimination

Research for new improved varieties, with high increase in yields and quality with disease resistance is required today to make mushroom production highly productive.Increasing the yield and quality of crops as well as resistance to diseases are the primary goals for mushroom breeders and mushroom research. Breeders now use DNA molecular markers to identify and select specific genes to locate s...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Applied sciences

سال: 2022

ISSN: ['2076-3417']

DOI: https://doi.org/10.3390/app12094754